Figure 5.

PNPase controls the stabilities of RsmY/Z. (A) Levels of the RsmY and RsmZ in indicated strains. Bacteria were grown to an OD₆₀₀ = 0.8 or 2 in LB. Total RNAs were collected and the relative levels of RsmY and RsmZ were determined with real time PCR. The RNA levels of proC were used as internal controls. ^*p < 0.05 compared to wild type PAK by Student's t-test. (B) Expression of RsmY and RsmZ in wild type PAK and the ΔKH-S1 mutant. Bacteria containing rsmY-lacZ or rsmZ-lacZ transcriptional fusion were grown to the similar OD₆₀₀ of 2.0 and subjected to β-galactosidase assays. Each assay was done in triplicates, and the error bars indicate standard deviations. ^*p < 0.05 compared to wild type PAK by Student's t-test. Degradation of RpsL (C), RsmY (D), and RsmZ (E) in wild type PAK and the ΔKH-S1 mutant. Bacterial cells with or without rifampicin treatment were spiked with equal numbers of gfp expressing E. coli cells. Total RNA was purified and the relative RNA levels were determined by real time PCR. The gfp RNA level in each sample was used as an internal control for normalization.