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. 2016 Jan;7(1-2):36–46. doi: 10.18632/genesandcancer.93

Figure 1. Restoration of factor independent growth of loss of function mutants of p185BCR/ABL by mutation T315I.

Figure 1

Modular organization of the p185BCR/ABL mutants+/− T315I. For the determination of factor-independent growth of loss of function mutatnts of p185BCR/ABL in the presence or absence of T315I mutation, 32D cells were retrovirally transduced with the indicated constructs. The number of viable cells was daily determined by Trypan blue dye-exclusion. The graphs show the means +/− SD of three independent experiments. A. p185BCR/ABL mutants with a point mutation at Y177 (Y177F) +/− T315I. B. p185BCR/ABL mutants lacking the CC oligomerization interface +/−T315I. C. p185BCR/ABL lacking the N-terminal CC-domain together with a point mutation at the Y177 (Y177F) +/−T315I. D. ΔS/T-p185 mutants where the N-terminus of BCR comprising the CC-domain and the Y177 phosphorylation site fused to the ABL-portion of the fusion protein +/−T315I. E. #ABL - the ABL-portion of the BCR/ABL fusion protein +/− T315I.