Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 2;6:22539. doi: 10.1038/srep22539

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Schematic representation of mutant Munc13-4 constructs. Domain organisation of Munc13-4 and amino acid positions corresponding to the truncation boundaries of each construct are shown. 1C2: the first Ca²⁺ -binding C2 domain, 2C2: the second Ca²⁺ -binding C2 domain, MHD1: the first Munc13 homology domain, and MHD2: the second Munc13 homology domain. (B) Identification of Rab37-interacting regions in Munc13-4 protein. Lysates from COS7 cells transfected with HA-Rab37DA or control (Vector) plasmid, together with the expression plasmid for FLAG-tagged Munc13-4 mutant protein presented in (A) were subjected to immunoprecipitation with anti-HA or anti-FLAG antibody as indicated. The lysates (Ly) and immunoprecipitates (IP) were subjected to western blot analyses with anti-HA (3F10) or anti-FLAG antibody. (C) Multiple Rab37 proteins interact with Munc13-4. COS7 cells were transfected with (+) or without (−) the following plasmids as indicated: FLAG-Rab37, HA-Munc13-4, and HA-Rab37 plasmids. The transfected cells were then lysed and subjected to immunoprecipitation with anti-FLAG antibody. The lysates (Ly) and immunoprecipitates (IP) were then analysed as in (B). (D) Formation of a Rab27-Munc13-4-Rab37 complex. COS7 cells were transfected with (+) or without (−) the following plasmids as indicated: FLAG-Rab27a, HA-Munc13-4, and HA-Rab37 plasmids. The transfected cells were then lysed in the presence of GTPγS and subjected to immunoprecipitation with anti-FLAG antibody. The lysates (Ly) and immunoprecipitates (IP) were then analysed as in (B). The full-length blotting images of (B−D) are presented in Supplementary Figs. S18, S20, and S21, respectively.