Figure 2.

Effects of LPA and EGF on signal transduction events in human breast cancer cells. (A) Serum-starved MCF-7 and MDA-MB-231 cells were incubated with 10 µM LPA or 10 nM EGF for 5 min. Whole-cell extracts were immunoblotted using antibodies recognizing phosphorylated active Erk and Akt. An immunoblot for total actin was performed as a loading control; (B) Serum-starved MCF-7 cells were incubated for the indicated times with 10 µM LPA or 10 nM EGF. Whole-cell extracts were immunoblotted using antibody recognizing total CCN1. An immunoblot for total actin was performed as a loading control. Each experiment is representative of at least three separate experiments.