Figure 6.

Dose-response for effects of FFAR agonists on breast cancer cell proliferation. Serum-starved MDA-MB-231 cells were incubated for 48 h with and without 10 µM LPA in the absence and presence of the indicated concentrations of EPA, GW9508, and TUG-891. The number of cells achieved in response to LPA alone was defined as 100% response; the number of cells present in the absence of LPA was defined as 0% response. Each point represents mean ± SEM (n ≥ 4 for values obtained from at least two experiments, each performed with two separate replicate wells of cells for each experimental condition.