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Formation of hydroxyl radicals as a function of catechins concentration in the presence of Cu(II). Reaction mixture (0.5 mL) contained 200 µg calf thymus DNA as substrate, 100 µM Cu(II) and indicated concentrations of C, EC, EGC and EGCG. The reaction mixture was incubated at 37 °C for 1 h. Hydroxyl radical formation was measured by determining the TBA reactive material. All values reported are means of three independent experiments. Error bars represent standard error of mean.
