Figure 4.

Time course of caspase-3 and -7 activation in H1437 cell line. Cells were treated with 27 μM of s-cal14.1a for 3, 6, 12 and 24 h. (A) Representative image showing untreated cells (C−) and cells treated with s-cal14.1a and C+ (staurosporine 1 μM) at 460× overall magnification; (B) Graph showing counting positive cells to caspase-3 and -7 activation. Cells stained blue were considered as 100%. Results were expressed as the percentage of cells that are positive to caspase-3 and -7 (green) and PI (red).