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. 2016 Mar 2;6:22451. doi: 10.1038/srep22451

Figure 4. Real-time qPCR to measure procyclin expression levels.

Figure 4

GUSone bloodstream forms were exposed for 24 hours to the 5 DIP compounds DIP-01, DIP-02, DIP-03, DIP-07 and DIP-19 and to CA at 37 °C. Procyclin mRNA levels were determined by qPCR. Among the standard trypanocidal drugs only melarsoprol induced expression of procyclin mRNA above background level. Error bars show standard deviations for technical triplicates.