Table 3. IC₅₀s in the Alamar blue viability assay using the T. b. brucei clone GUSone with different exposure times from 16 h to 48 h.

Compound	IC50[μg/ml]				MIC [μg/ml]
	16 h	24 h	40 h	48 h	24 h
DIP-01	75.6	70.1	57.2	47.1	>100
DIP-02	1.2	1.1	1.1	1.2	10
DIP-03	0.4	0.4	0.4	0.4	2.5
DIP-07	2.2	2.0	2.2	1.9	20
DIP-19	0.9	0.9	0.8	0.8	20
Melarsoprol	0.021	0.020	0.016	0.012	0.25
Pentamidine	0.018	0.012	0.006	0.003	>20
CA	>20 mM	>20 mM	20 mM	8 mM	>20 mM

Minimum Concentration to kill all trypanosomes (MIC) was determined microscopically after 24 h of exposure. Assays were performed starting with 4 × 10⁵ trypanosomes in 0.5 ml. CA: cis-aconitate 20 mM = 3.48 mg/ml.