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CCR2 inhibition decreased the number of macrophages that were double positive for iNOS and F4/80 and CD206 and F4/80 in the stenotic kidney with no change in F4/80 and arginase 1 double-positive macrophages. The extent of macrophage infiltration is shown as assessed by counting the number of double-positive macrophages for F4/80 and iNOS (A), F4/80 and arginase 1 (B), and F4/80 and CD206 (C) stain in 10–15 random fields over the whole cortical region.
