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Immunofluorescence staining for double-positive macrophages showing expression of F4/80 and inducible nitric oxide synthase (iNOS). A: immunofluorescence image showing staining for 4′,6-diamidino-2-phenylindole (DAPI; blue), F4/80 (green), and iNOS (red). Double-positive macrophages expressing F4/80 and iNOS are pointed out with white arrows. B: number of F4/80 and iNOS double-positive cells at ×400 magnification. *P = 0.05. **P = 0.001.
