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. 2015 Dec 9;310(5):F372–F384. doi: 10.1152/ajprenal.00131.2015

Fig. 5.

Fig. 5.

Renal parenchymal cells were responsible for early Ccl2 expression post-RAS. A: representative histological images of stenotic kidneys of CCL2-RFP reporter mice with RAS surgery as stained with antibody against red fluorescent protein (RFP) at 24 h, 3 days, and 7 days postsurgery compared with mice 7 days post-sham surgery. All images were taken at ×400 magnification. Scale bar = 100 μm. B: expression of Ccl2 as assessed by RT-PCR in the stenotic kidneys of mice with RAS (n = 13–20 mice for each time point) or sham surgery (n = 10 mice for each time point). C: gene expression of Ccl2 in CD11b+- and CD11b−/CD90− (kidney parenchymal cells)-enriched fractions isolated from the stenotic kidney of mice with RAS (n = 4–5 mice for each time point) or sham surgery (n = 4–5 mice for each time point) at 3 and 7 days postsurgery. Values are means ± SE normalized against 18S transcript and expressed as fold-increase relative to the 7-day sham expression. *P < 0.05, **P = 0.001, ***P = 0.0001 compared with respective sham.