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. 2015 Dec 18;310(5):L403–L414. doi: 10.1152/ajplung.00259.2015

Fig. 2.

Fig. 2.

Inhibition of D110C-CFTR and D110C/K892C-CFTR by cadmium demonstrated differences in reversibility. Traces show change in macroscopic current (μA) over time during activation by stimulation of coexpressed β2AR using isoproterenol. All cells were preincubated with 1 mM DTT for 5 min. A–D: D110C/K892C-CFTR, D110C-CFTR, WT-CFTR, and K892C-CFTR, all with 30-s exposures to 20 μM cadmium followed by 30 s of cadmium washout in the presence of isoproterenol. Current was reported at Vm = −40 mV; increased activation results in downward deflection (n = 4–7 per variant). E: D110C-CFTR and D110C/K892C-CFTR percent current recovery after 30 s of cadmium washout in the continuing presence of isoproterenol. *P < 0.05.