Fig. 4.
Neither 1 mM DTT nor 20 μM cadmium significantly affected the function of other extracellular loop 1/4 (ECL1/ECL4) cysteine mutants. A: trace showing that neither 1 mM DTT nor 20 μM cadmium significantly affected E115C/K892C-CFTR. B: inhibition of current by cadmium at Vm = −60 mV after 60 s of treatment. *P < 0.05. C: potentiation of current at Vm = −60 mV by 1 mM DTT after 3 min of treatment. D: snapshot of a molecular dynamics simulation at 2.5 ns from our recent study (32), with D110, D112, E115, and K892 highlighted. Orientation of ECL1 is such that D112 and E115 are farther from K892 than is D110.