Figure 3. Percentages of viability and total motility of boar sperm cells subjected to ‘in vitro’ capacitation and subsequent progesterone-induced ‘in vitro’ acrosome exocytosis after photo-stimulation procedures.

Boar sperm were subjected to separate photo-stimulation procedures and the subsequent IVC/IVAE as described in the Methods section. At the indicated times, aliquots were taken and percentages of viability (a) and total motility (b) were determined. 0: values at 0 h of incubation in the capacitation medium (CM). 4: values at 4 h of incubation in the CM. 5: values after 4 h IVC plus 60 min of the progesterone-induced IVAE. Results are shown as mean ± SEM for 12 separate experiments. Asterisks indicate significant (P < 0.05) differences when compared with the respective Control value at the same time point.