. 2016 Mar 2;6:22569. doi: 10.1038/srep22569

Table 1. Motility parameters of boar sperm subjected to separate photo-stimulation procedures and subsequently incubated at 37 °C for 90 min.

	Time	VCL (μm·s⁻¹)	VSL (μm·s⁻¹)	VAP (μm·s⁻¹)	LIN (%)	STR (%)	WOB (%)	ALH (μm)	BCF (Hz)
Control	0 min	62.6 ± 3.4^a	25.5 ± 1.6^a	38.6 ± 2.1^a	42.4 ± 2.4^a	65.7 ± 2.6^a	61.7 ± 1.5^a	2.7 ± 0.1^a	7.8 ± 0.2^a
	15 min	38.4 ± 2.5^b	23.8 ± 1.0^a	30.3 ± 1.3^b	63.6 ± 2.1^b	78.2 ± 1.7^b	79.2 ± 1.8^b	1.6 ± 0.1^b	7.3 ± 0.2^a
	30 min	37.3 ± 1.2^b	26.2 ± 1.6^a	30.2 ± 1.2^b	71.5 ± 2.6^c	85.3 ± 1.8^c	81.7 ± 1.6^bc	1.6 ± 0.1^b	6.8±0.2^b
	60 min	41.3 ± 1.9^b	29.1 ± 1.5^a	33.9 ± 1.7^b	73.7 ± 2.0^c	87.3 ± 1.7^c	83.2 ± 1.4^c	1.3 ± 0.1^b	7.6 ± 0.3^a
	90 min	42.3 ± 2.0^b	27.3 ± 1.8^a	33.5 ± 2.1^b	64.7 ± 3.7^b	76.4 ± 2.7^b	80.9 ± 2.7^bc	1.4 ± 0.1^b	7.2 ± 0.2^a
Procedure #1 (10-10-10)	0 min	49.6 ± 2.3ª^*	21.6 ± 1.7^a	31.2 ± 1.5^a*	46.4 ± 2.4^a	71.8 ± 3.0^b	63.2 ± 1.3^a	2.1 ± 0.1ª*	9.2 ± 0.3^a*
	15 min	37.5 ± 2.4^b	21.1 ± 1.5^a	27.6 ± 1.9^a	61.9 ± 2.1^b	76.5 ± 1.2^b	79.2 ± 1.2^b	1.6 ± 0.1^b	7.2 ± 0.3^b
	30 min	44.3 ± 3.9ª^*	34.4 ± 3.4^b*	38.1 ± 3.0^b*	79.0 ± 1.8^c*	90.2 ± 1.5^c	87.6 ± 1.1^c*	1.6 ± 0.1^a	7.2 ± 0.2^b
	60 min	47.0 ± 3.0^a*	32.2 ± 2.0^b	38.0 ± 2.4^b*	73.2 ± 1.9^c	86.3 ± 1.4^c	84.0 ± 1.1^c	1.5 ± 0.1^b	7.8 ± 0.2^b
	90 min	47.6 ± 1.3^a*	33.9 ± 1.4^b*	38.9 ± 0.9^b*	76.9 ± 1.1^c*	83.7 ± 1.4^c*	86.4 ± 2.5^c	1.8 ± 0.1^a	9.1 ± 0.3^a*
Procedure #2 (15-10-15)	0 min	63.3 ± 3.6^a	27.0 ± 2.4^a	41.5 ± 2.3^a	44.1 ± 3.0^a	66.1 ± 2.9^a	64.2 ± 1.3^a	2.5 ± 0.2^a	8.6 ± 0.4^a*
	15 min	43.7 ± 3.9^bc	24.5 ± 1.9^a	31.8 ± 2.3^b	60.2 ± 2.0^b	76.2 ± 1.2^b	77.2 ± 1.9^b	2.0 ± 0.1^b*	8.3 ± 0.3^a*
	30 min	37.1 ± 2.0^b	25.7 ± 1.1^a	31.0 ± 1.3^b	71.2 ± 1.1^c	82.8 ± 1.3^c*	84.3 ± 0.9^c	1.5 ± 0.1^c	6.5 ± 0.5^b
	60 min	38.8 ± 1.1^b	29.9 ± 1.7^a	33.2 ± 1.5^b	73.8 ± 1.4^c	86.5 ± 1.3^d	84.8 ± 1.2^c	1.4 ± 0.1^c	7.9 ± 0.3^a
	90 min	49.1 ± 2.7^c*	36.8 ± 2.9^b*	39.5 ± 2.2^a*	80.8 ± 3.6^d*	92.8 ± 3.0^e*	89.1 ± 2.0^d*	1.7 ± 0.1^c	9.6 ± 0.5^b*
Procedure #3 (20-10-20)	0 min	66.3 ± 2.2^a	23.9 ± 1.4^a	36.6 ± 1.3^a	43.1 ± 1.5^a	62.4 ± 3.3^a	61.1 ± 1.1^a	2.5 ± 0.1^a	8.7 ± 0.5^a*
	15 min	40.3 ± 2.2^b	29.9 ± 1.9^b*	31.4 ± 1.8^b	74.5 ± 2.2^b*	86.7 ± 1.4^b*	84.4 ± 2.5^b	1.6 ± 0.1^b	7.4 ± 0.2^b
	30 min	35.9 ± 1.6^b	23.8 ± 1.6^a	27.9 ± 1.5^b	69.5 ± 1.1^c	84.3 ± 1.8^b	79.7 ± 1.3^b	1.6 ± 0.0^b	6.6 ± 0.5^b
	60 min	48.7 ± 2.0^c*	22.0 ± 1.2^a*	32.3 ± 1.8^b	49.2 ± 3.4^d*	70.7 ± 2.6^c*	76.9 ± 2.6^bc*	1.9 ± 0.1^c*	7.4 ± 0.3^b
	90 min	51.2 ± 2.1^c*	30.8 ± 1.2^b	38.3 ± 1.0^a*	68.4 ± 1.9^c	87.0 ± 1.9^b*	74.8 ± 1.9^c*	2.3 ± 0.1^c*	6.8 ± 0.4^b

Boar spermatozoa were subjected to separate photo-stimulation procedures and subsequent incubation at 37 °C as described in the Methods section. At the indicated times, samples were taken and CASA-obtained motility parameters were registered. Both motility parameters and statistical procedures have been described in the Methods section. Results are shown as mean ± SEM. for 12 separate experiments. Different superscript letters indicate significant (P < 0.05) differences when compared with the respective 0-min point. Asterisks indicate significant (P < 0.05) differences when compared with the respective Control value at the same time point.