. 2016 Mar 2;6:22569. doi: 10.1038/srep22569

Table 2. Motility parameters of boar sperm subjected to ‘in vitro’ capacitation and further ‘in vitro’ progesterone-induced acrosome exocytosis after being treated with separate photo-stimulation procedures.

	Time	VCL (μm·s⁻¹)	VSL (μm·s⁻¹)	VAP (μm·s⁻¹)	LIN (%)	STR (%)	WOB (%)	ALH (μm)	BCF (Hz)
Control	0 h	61.0 ± 3.7^a	23.2 ± 2.2^a	34.6 ± 2.8^a	37.3 ± 1.7^a	62.3 ± 1.8^a	56.6 ± 1.1^a	2.6 ± 0.2^a	5.8 ± 0.5^a
	4 h	69.2 ± 3.3^b	32.1 ± 3.1^b	46.8 ± 2.4^b	59.9 ± 2.7^b	76.0 ± 4.0^b	61.8 ± 2.4^b	2.3 ± 0.2^a	6.5 ± 0.3^b
	5 h	79.1 ± 6.1^c	35.4 ± 2.3^b	42.1 ± 2.9^b	62.9 ± 3.8^b	90.0 ± 3.2^c	62.0 ± 3.5^b	3.4 ± 0.3^b	6.2 ± 0.2^b
Procedure #1 (10-10-10)	0 h	70.8 ± 2.8^a*	23.6 ± 2.7^a	38.6 ± 2.8^a	32.0 ± 2.7^a*	56.2 ± 3.8^a	52.7 ± 1.7^a	3.3 ± 0.2^a*	5.1 ± 0.6^a
	4 h	99.9 ± 4.9^b*	30.7 ± 3.6^b	47.5 ± 3.8^b	47.5 ± 3.8^b*	68.3 ± 4.6^b	54.7 ± 2.4^a	3.0 ± 0.3^a*	6.7 ± 0.3^b
	5 h	93.3 ± 2.0^b*	67.1 ± 3.5^c*	83.2 ± 1.8^c*	92.4 ± 5.9^c*	86.9 ± 4.7^c	84.1 ± 4.2^b*	2.3 ± 0.0^b*	8.6 ± 0.7^c*
Procedure #2 (15-10-15)	0 h	63.3 ± 2.8^a	23.5 ± 2.5^a	34.0 ± 2.6^a	38.2 ± 2.0^a	63.3 ± 2.0^a	55.8 ± 2.1^a	2.6 ± 0.2^a	5.5 ± 0.2^a
	4 h	81.5 ± 3.1^b*	30.2 ± 2.6^b	46.2 ± 2.5^b	51.0 ± 5.0^b	81.5 ± 4.9^b	56.9 ± 4.0^a	2.5 ± 0.1^a	6.6 ± 0.4^b
	5 h	61.2 ± 3.4^a*	30.9 ± 2.8^b	48.2 ± 3.5^b	70.7 ± 3.2^b	92.6 ± 2.7^c	69.7 ± 3.5^b	2.2 ± 0.1^b*	6.7 ± 0.3^b
Procedure #3 (20-10-20)	0 h	62.6 ± 3.6^a	23.1 ± 2.6^a	38.4 ± 2.9^a	39.1 ± 2.6^a	57.1 ± 3.6^a	52.1 ± 2.6^a	3.3 ± 0.2^a*	5.1 ± 0.6^a
	4 h	56.8 ± 3.5^b*	24.8 ± 3.4^a*	36.1 ± 2.9^a*	43.2 ± 4.6^a*	65.2 ± 4.1^b*	60.6 ± 2.9^b	2.5 ± 0.1^b	6.3 ± 0.5^b
	5 h	49.4 ± 4.0^c*	14.9 ± 1.2^b*	27.0 ± 2.7^b*	33.0 ± 1.1^b*	55.7 ± 3.1^a*	54.2 ± 1.8^a*	2.3 ± 0.2^b*	6.9 ± 0.4^b

Boar spermatozoa were subjected to separate photo-stimulation procedures and subsequent ‘in vitro’ capacitation and further progesterone-induced ‘in vitro’ acrosome exocytosis as described in the Methods section. At the indicated times, samples were taken and CASA-obtained motility parameters were registered. Both motility parameters and statistical procedures have been described in the Methods section. Results are shown as mean ± SEM for 12 separate experiments. Different superscript letters indicate significant (P < 0.05) differences when compared with the respective 0-h point. Asterisks indicate significant (P < 0.05) differences when compared with the respective Control value at the same time point 0 h. Values of sperm taken at 0 h of incubation in the capacitation medium (CM). 4 h: Values of sperm taken at 4 h of incubation in the CM. 5 h: Values of sperm taken after 4 h of IVC plus 60 min of the progesterone-induced IVAE.