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. 2016 Mar 2;6:22581. doi: 10.1038/srep22581

Figure 3. Identification of the boundary of miR-15b promoter region.

Figure 3

(a) The structure of the human DNA loci expressing miR-15b is shown. miR-15b and miR-16-2 cluster resides on chromosome 3 in intron 5 of host SMC4 gene. TSS, transcriptional start site. (b–d) HeLa cells were infected with JEV at an MOI of 1 for the indicated times. qRT-PCR was performed to measure the expression of SMC4 (b), pre-miR-16-2 (c) and miR-16-2 (d). The non-infected cells were used as controls and values from these cells were regarded as 1. (e) Hela cells were transfected with miR-15b promoter reporters containing various lengths of the miR-15b promoter region for 24 h and then either left uninfected or infected with JEV at an MOI of 1. 24 h later, luciferase activity was measured, and results were expressed as percentages of TSS-2518 basal activity (regarded as 100%). Similar results were obtained in three independent experiments. Statistical analysis was carried out by 2-way ANOVA with subsequent t tests using a Bonferroni post-tests. *p < 0.05, **p < 0.01, ***p < 0.001.