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. 2016 Mar 2;6:22581. doi: 10.1038/srep22581

Table 2. Primers used for ChIP and qRT-PCR.

Primer Name Application Sequence 5′-3′
miR-15b promoter (ChIP) F ChIP AACCCTTTTCTCACCAGGTCCA
miR-15b promoter (ChIP) R TTCAGAAGAGGGCGGGCTC
pri-miR-15b F qRT-PCR CTTCTGTCTATCACATAAGTGG
pri-miR-15b R GGTCCAAGTCAATTCCATG
pre-miR-15b F GGCCTTAAAGTACTGTAGC
pre-miR-15b R CCTTAAATTTCTAGAGCAGC
pre-miR-16-2 F CTTGTTCCACTCTAGCAGCAC
pre-miR-16-2 R GTCACACTAAAGCAGCACAGTA
miR-16-2 F TAGCAGCACGTAAATATTGGCG
miR-16-2 R GTGCAGGGTCCGAGGT
SMC4 F ACGGCATAGTGAACGAGAAA
SMC4 R GTCAGGGGCTGTAGCAAGTA
RIG-I F TTCCCACAAGGACAAAAG
RIG-I R GCCTGTAACTCTATACCCAT
TLR3 F TCCCAAGCCTTCAACGACTG
TLR3 R GGGTTTGCGTGTTTCCAGAG
β-actin F AGCGGGAAATCGTGCGTGAC
β-actin R GGAAGGAAGGCTGGAAGAGTG