Figure 3. The effects of yeast feeding treatment on adult activity levels are species specific.

(a) Cladogram of melanogaster and suzukii subgroups. Only the species tested were included in the cladogram. (b) Relative locomotor activity levels in D. suzukii, D. melanogaster, D. simulans, and D. biarmipes following a three-day treatment with yeast expressing dsRNA against yTub23C. Activity was recorded using the DAMS during the 4 days following the treatment. Data represent averages and s.e.m. of 3 independent experiments, and a total of 96 flies were tested per treatment. Each replicate was normalized to the average of the control treatment. One-way ANOVA was performed (p = 0.0001) and significance relative to the control was determined by post hoc Dunnett corrected t-test (***indicates p < 0.001). (c) Activity levels plotted over the second day of recording following three days of feeding treatment. Each bar represents average activity counts in 15-minute increments. Black bars indicate lights off and grey bars indicate lights on. Presence or absence of light as well as the timing of light transition are also indicated in the horizontal bar underneath each activity graph; light turns on at 6am and turns off at 6pm each day of recording. Each eduction graph represents an average of 32 flies in one experiment. Representative eduction graphs are shown here.