Figure 2. Accumulation of macrophage-like cells in FV3-infected tadpoles’ brain tissues.
Cryosections (8 μm) of tadpole hindbrains 6 days post-FV3 infection were fixed with 4% cold paraformaldehyde then stained with X. laevis specific anti-class II AM20 or mouse anti-HAM56 mAbs followed by Dylight 594-conjugated F (ab’)2 donkey anti-mouse IgG (H + L) (Jackson Immuno Research, PA). Cellular nuclei were stained with the DNA intercalator Hoechst-33258. Sections were mounted in anti-fade medium (Molecular Probes, Oregon) and visualized with a fluorescence microscope using an Axiovert 200 inverted fluorescence microscope and Infinity 2 digital camera (objectives x5/x10/x20; Zeiss). Digital images were analyzed and processed by ImageJ software from NIH.