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. 2016 Mar 2;6:22508. doi: 10.1038/srep22508

Figure 7. Infiltration of infected leukocytes and dissemination of FV3 in the tadpole’s brain.

Figure 7

Mock-infected or 6 d.p.i FV3 pre-metamorphic inbred J tadpoles were adoptively transferred by intracardiac injection of 100,000 (10 μL volume) uninfected or in vitro FV3-infected (1 d.p.i) PLs previously labeled with 2 μM red fluorescent membrane PKH26. One day post-transfer, tadpole recipients were anesthetized and infiltration of leukocytes into the tadpole’s brain was visualized and quantified by fluorescence microscopy analysis of whole mount brain. (A) Results are representative of 3 replicates and displayed as means ± SE from 10 animals from uninfected tadpole recipients (0 dpi) injected with either uninfected (black bar) or infected (gray bar) PLs; or from infected tadpoles recipients at (6 dpi) injected either with uninfected (black bar) or infected (gray bar) PLs. *P < 0.05 and **P < 0.01 significant differences relative to uninfected tadpole’s brain using one-way ANOVA test and Tukey post hoc test. (B) FV3 genome copy number determined by absolute qPCR using primer specific for FV3 vDNAPol.