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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Adv Microb Physiol. 2014;64:115–143. doi: 10.1016/B978-0-12-800143-1.00003-8

Table 2.

List of RNA-i mutants constructed and the previously published phenotypes.

Gene ID Protein Growth reduction/Phenotype Ref.
Polyamine pathway
Tb09.v1.0380 Spermidine synthase Spermidine auxotrophy, depletion of T(SH)2 and cell death [48]
Tb11.01.5300 Ornithine decarboxylase Depletion of reduced thiols and cell growth arrest [49]
Tb927.6.4410 S-adenosylmethionine decarboxylase 60-90% [50]
Tb927.6.4470 Prozyme, activator of SAMDC 100% [50]
Tb927.6.4840 Methionine adenosyltransferase No effect [50]
Tb927.6.4890 S-adenosylmethionine synthase Unknown [50]
Tb927.8.1910 Acetylornithine deacetylase No effect [50]
Pentose Phosphate Pathway and related enzymes
Tb927.10.2490 Glucose-6-phosphate 1-dehydrogenase Growth arrest, cell death [46]
Tb11.02.4200 6-phosphogluconolactonase None [50]
Tb09.211.3180 6-phosphogluconate dehydrogenase 70-100% [50], *
Tb927.8.6170 Transketolase None [50]
Tb927.10.12210 Ribulose-5-phosphate 3-epimerase None [50]
Tb11.03.0090 Ribokinase 70-80% [50]
Tb927.4.1350 Glyoxalase II 20-80% [50, 51]
Tb11.01.0700 Ribose 5-phosphate isomerase >90% [50]
Redox metabolism
Tb927.3.3760 Tryparedoxin Growth arrest and enhanced sensitivity against hydrogen peroxide [50, 52]
Tb09.160.2020 Thioredoxin None [50, 53]
Tb927.2.4370 Trypanothione synthetase Depletion of Gsp and T(SH)2, growth arrest, loss of viability, enhanced sensitivity against hydroperoxides and drugs [32, 54]
Tb927.7.4000 Glutathione synthetase 10-80% [50]
Tb927.10.12370 Gamma-glutamylcysteine synthetase Depletion of GSH and T(SH)2, cell death and loss of virulence [55]
Tb927.10.10390 Trypanothione reductase Growth arrest, loss of viability and virulence [56]
Tb11.01.7560 Glutathione peroxidase, putative >90% [50]
Tb11.12.0016 Glutathionylspermidine synthetase No effect [50]
Tb09.160.4250 Peroxiredoxin-type tryparedoxin peroxidase (cytosolic) Loss of viability and enhanced sensitivity against hydrogen peroxide [50, 57]
Tb927.4.2450 Protein disulfide isomerase I 15-60% [50]
Tb927.3.4240 Thioredoxin, putative 40-80% [50]
Tb927.5.950 Monothiol glutaredoxin 3 50-100% [50]
Tb927.8.1990 Peroxiredoxin-type tryparedoxin peroxidase (mitochondrial) None [50, 57]
*

= Kerkhoven, Achcar et al., under review