Fig. 7. Upregulation of protein kinase C, delta in Hdac1^Δ/+nHdac2^Δ/Δn mice.

(A) Relative mRNA expression of Prkcd in P0 Hdac1^Δ/+nHdac2^Δ/Δn brains (light gray) compared with the corresponding wild-type littermate controls (black). Values are normalized to the housekeeping gene Gapdh. Error bars indicate s.d. (n=4). **P<0.01. (B) Quantification of PKCδ protein levels in P0 Hdac1^Δ/+nHdac2^Δ/Δn brains (light gray) compared with the corresponding wild-type littermate controls (black). Immunoblot signals were scanned using ImageQuant Software and values are normalized to β-actin. Error bars indicate s.d. (n=4). **P<0.01. (C) Immunoblot analysis of P0 wild-type littermate controls versus Hdac1^Δ/+nHdac2^Δ/Δn brain extracts with antibodies against HDAC1, HDAC2, PKCδ and β-actin as loading control. (D) Immunoblot analysis of P0 wild-type littermate control versus Hdac1^Δ/+nHdac2^Δ/Δn brain extracts with antibodies against pPKC substrate and β-actin as loading control. (E) Schematic representation of the Prkcd gene with exons depicted as numbered black boxes. Primers used for the chromatin-immunoprecipitation experiment are illustrated as gray rectangles. (F-H) Chromatin from littermate control and Hdac1^Δ/+nHdac2^Δ/Δn brains was immunoprecipitated with antibodies specific for HDAC1 (F), HDAC2 (G), H3K9ac (H) (white bars) and IgG as negative control (black bars) followed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) with primers specific for different regions of the Prkcd gene as illustrated in E. Error bars indicate s.d. (n≥2). TSS, transcriptional start site.