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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Virology. 2016 Feb 6;490:83–90. doi: 10.1016/j.virol.2016.01.009

Table 3.

SNPs at attenuating mutations E2-12 and E2-82 in CHIKV-7 and 181/25 viruses*.

E2-12 8575 8576 8577
CHIKV-7 A U A
Count % Count % Count %
Total 32737 100.000 32820 100.000 32474 100.000
A 32689 99.853 45 0.137 32426 99.852
C 17 0.052 graphic file with name nihms758007t1.jpg 10 0.031
G 16 0.049 22 0.067 31 0.095
U 14 0.043 32729 99.723 7 0.022
N 1 0.003 3 0.009 0 0.000
181/25
Total 46481 100.000 46351 100.000 45665 100.000
A 46332 99.679 242 0.522 45540 99.726
C 38 0.082 graphic file with name nihms758007t2.jpg 29 0.064
G 77 0.166 136 0.293 75 0.164
U 30 0.065 45885 98.995 17 0.037
N 4 0.009 5 0.011 4 0.009
E2-82 8785 8786 8787
CHIKV-7 A G G
Count % Count % Count %
Total 33856 33703 33551
A 33789 99.802 8 0.024 5 0.015
C 16 0.047 7 0.021 6 0.018
G graphic file with name nihms758007t3.jpg 33654 99.855 33478 99.782
U 22 0.065 34 0.101 62 0.185
N 0 0.000 0 0.000 0 0.000
181/25
Total 55660 55573 56070
A 55494 99.702 40 0.072 29 0.052
C 40 0.072 23 0.041 45 0.080
G graphic file with name nihms758007t4.jpg 55372 99.638 55818 99.551
U 42 0.075 130 0.234 174 0.310
N 10 0.018 8 0.014 4 0.007
*

Amino acid position E2-12: the AUA codon encodes attenuated Ile, while ACA codon encodes reversion mutation to the wild-type Thr. Amino acid position E2-82: the AGG codon encodes attenuated Arg, while GGG codon encodes reversion mutation to the wild-type Gly. Columns show nucleotides at indicated genomic positions. Rows indicate frequencies of SNPs for each possible nucleotide. Reversion SNPs are boxed.