Figure 1.

SDS-PAGE analysis of ferritin distribution between soluble and aggregation after bacterial cell ultrasonic disruption.

Note. Ferritin was overexpressed in the E. coli BL21 (DE3) strain. 5 mediums were studied as ultrasonic buffer: LB medium, lysis buffer, 1%Typone, 5%Yeast Extract and 1%NaCl. Here the soluble and aggregation of ferritin can be abserved. Higher percentage of the aggregates can be observed in the insoluble sample after ultrasonic disruption (n=3). LB medium-insoluble form after sonication. Lysis buffer-soluble form after sonication. 1% Typone- soluble form after sonication. 5% Yeast Extract-soluble form after sonication. 1%NaCl- insoluble form after sonication. S-supernatant & P-precipitate.