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Results of critical threshold from 4 measurements at each PCR location with identical concentration of the HA gene. The graphical output is shown in Figure 5B. The discrepancy between results from individual samples was probably caused by sample misalignment with respect to the heater as they were placed manually.
| Position | Mean CT | Standard deviation | Concentration HA (ng/μL) |
|---|---|---|---|
| 1 | 9.6 | 1.5 | ≈ 5.0×10−5 |
| 2 | 9.0 | 1.2 | ≈ 5.0×10−5 |
| 3 | 9.5 | 0.6 | ≈ 5.0×10−5 |
| 4 | 8.0 | 0.8 | ≈ 5.0×10−5 |
