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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Am J Transplant. 2015 Mar 17;15(5):1253–1266. doi: 10.1111/ajt.13113

Figure 1. Characteristics of expanded cynomolgus monkey Treg and Teff.

Figure 1

(A) Expanded Treg (black lines) and Teff (grey shading) were stained for Foxp3, CD25, CD39, CD45RA, CD62L, CD95, CD127, Bcl-2, Helios, CTLA-4, CCR7 and CXCR3 at the end of round 3 of expansion. Compared to expanded Teff, expanded Treg showed much higher expression of Foxp3, Helios, CD39 and CXCR3, and similar high expression of CD25. Data are representative of 3 experiments. (B) Demethylation status of Foxp3 TSDR (Treg-specific demethylation region) in fresh and expanded Treg and Teff, assessed at the end of the 2nd and 3rd round of expansion. The % TSDR demethylation in each experiment is shown, as well as the mean % for all experiments in each group (horizontal line). TSDR demethylation was significantly higher (at least 4-fold) in Treg than in Teff under each condition. ***, p<0.001; *, p<0.05.