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. Author manuscript; available in PMC: 2016 Dec 18.
Published in final edited form as: ACS Chem Biol. 2015 Oct 28;10(12):2891–2899. doi: 10.1021/acschembio.5b00592

Figure 3.

Figure 3

The On-Bead Two-Color (OBTC) combinatorial cell screen. (a) Schematic representation of the assay. One-bead one-compound library beads (large blue circles) were treated with 1:1 mixture of red and green quantum dot stained HCC4017 cells and HBEC30KT cells respectively. A bead bound only by red cells indicates that the compound on that bead recognized a biomolecule uniquely present on HCC4017 cell surface that is absent (or negligible) in HBEC30KT cell surface. (b & c) Fluorescent microscopic images of beads; (b) One of the three beads found with only red stained HCC4017 cells bound. (c) A bead bound to both red and green stained cells (non-specifics). (d) The structure of PPS1 identified from the screen.