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. Author manuscript; available in PMC: 2016 Dec 18.
Published in final edited form as: ACS Chem Biol. 2015 Oct 28;10(12):2891–2899. doi: 10.1021/acschembio.5b00592

Figure 4.

Figure 4

Qualitative binding and specificity of the PPS1. PPS1 containing tentagel beads were exposed to: (a) Red stained HCC4017 cells alone, (b) Green stained HBEC30KT cells alone, (c) Red and green stained cells at 1:1 mixture. PPS1 predominantly bound to red stained HCC4017 cells over HBEC30KT cells. (d) Red stained HCC4017 cells did not bind to tentagel beads carrying scrambled version PC2. (E) Structure of the c-terminal biotinylated PPS1. (e) Streptavidin-magnetic beads coated with biotinylated PPS1 pulled down only HCC4017, but not HBEC30KT or HBEC3KT cells. The magnetic beads alone or control PC462 compound coated magnetic beads fail to pulldown HCC4017 cells.