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. Author manuscript; available in PMC: 2016 Dec 18.
Published in final edited form as: ACS Chem Biol. 2015 Oct 28;10(12):2891–2899. doi: 10.1021/acschembio.5b00592

Figure 5.

Figure 5

ELISA-like quantitative binding and specificity validation of the PPS1. (a) Chemical structure of c-terminus fluorescein isothiocyanate (FITC) labelled PPS1 (FITC-PPS1). (b) Binding curve of HCC4017 cells with PPS1-FITC indicates KD around 5μM. (c) Chemical structure of N-terminus modified Eu3+-chelated DTPA labelled PPS1. (d) Binding curve of HCC4017 cells with PPS1-(Eu3+)-DTPA indicates KD around 5-7μM.