Fig. 2.

Certolizumab pegol (CZP) induces heme oxygenase 1 (HO-1) expression. Monocytes were incubated, or not, with CZP (5 μg/ml) for 4 h of culture. RNA was isolated and qRT-PCR was performed to quantify HO-1 mRNA. Quantification of five experiments. *Paired t test <0.05 (a). Monocytes were incubated, or not, with CZP (5 μg/ml) during 6 or 16 h of culture. HO-1 protein was assessed by western blot (b). Quantification of five experiments was performed at 6h (c) and at 16h (d). Paired t test <0.05. GAPDH glyceraldehyde 3-phosphate dehydrogenase