Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 2;6:22378. doi: 10.1038/srep22378

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Western blot of CypA in Ppia^+/+ and Ppia^−/− primary osteoblastic cells. GAPDH was used as a loading control. (b) ALP, OCN and Runx2 expression in Ppia^+/+ and Ppia^−/− osteoblastic cells after seven days of osteogenic differentiation. (c) ALP and Alizarin red staining in Ppia^+/+ and Ppia^−/− osteoblastic cells after seven and 21 days of osteogenic differentiation, respectively. (d) CypA levels assayed in MC3T3-E1 osteoblastic cells after silencing or forced overexpression. Two clones for CypA silencing are referred to as M1 and M3, while two clones for overexpression are designated P1 and P2 (comparison include scrambled control, SC, and wildtype parental cells, WT). (e) ALP, OCN and Runx2 expression in WT, SC, M1, M3, P1 and P2 MC3T3-E1 cells after seven days of osteogenic differentiation. (f) ALP and Alizarin red staining in WT, SC, M1, M3, P1 and P2 MC3T3-E1 cells during osteogenic differentiation. (g) Expression of CypA in Ppia^+/+ and Ppia^−/− mouse primary osteoclastic cells. (h) TRAP staining of Ppia^+/+ and Ppia^−/− osteoclast cells after seven days of osteoclastic induction. (i) TRAP and Cathepsin K expression in Ppia^+/+ and Ppia^−/− osteoclastic cells after four and eight days osteoclastic induction. Magnification: 10 ×. (j) CypA levels assayed in RAW264.7 osteoclastic cells after silencing or forced overexpression. Two clones for CypA silencing are referred to as R1 and R2, while two clones for overexpression are designated P1 and P2 (comparison include scrambled control, SC, and wildtype parental cells, WT). (k) TRAP staining in (j). Magnification: 10×. (l) TRAP and Cathepsin K expression in WT, SC, R1, R2, P1 and P2 RAW264.7 osteoclastic cells after four and eight days osteoclastic induction. Data represented as mean and SD of three experiments of each group. (m) Resorptive activity assay in WT, SC, R1 and R2 RAW264.7 cells after seven days of osteoclastic induction. Resorptive areas are highlighted in red at 10× magnification. *P < 0.05 compared to Ppia^+/+ or WT cells.