Table 1.

Schedule of data collection, assessments and laboratory analyses in mothers and offspring in the PREOBE-study

Measure	Outcomes	Time of collection
Baseline recruitment information	Pre-gestational BMI, ethnicity, medical history, age, education, parity, smoking, ongoing supplements	Recruitment visit, 24 wk, and 34 wk
Maternal anthropometrics	Weight, length, circumference of abdomen, waist etc., skinfold thickness of triceps, subscapularis etc.a	24 wk, 34 wk
Bioimpedance	BMRa, impedancea, BMIa, FM/FMIa, FFM/FFMIa, body watera	24 wk, 34 wk
Fetal ultrasound	Fetal anthropometricsa	24 wk, 34 wk
Urine samples	Metabolomicsa	24 wk, 34 wk
Fecal samples	Microbiotab, metabolomicsa	24 wk, near delivery
Cheek cell sample	Microbiotaa, geneticsab, fatty acidsa	34 wk (mother), birth (infant)
Food questionnaire	Quantitative and qualitative dietary analysesa	24 wk, 34 wk
Placenta samples	Placental weight and circumference, Lipid profilea, phospholipidsa, gene expressiona, lipid peroxidation, pTfRb	Delivery
Lifestyle questionnaire	Lifestyle and physical activitya	24 wk
Blood samples (Mother and cord blood)	Direct analyses: ESR, glucose, lipidsa, urea, creatinine, uric acid, bilirubin, iron status, folate, TSH, amylase, CRP, HbA1c, total protein, albumin, vit B12, insulin, cortisol, IGF1a, IGFBP1a, and lymphocyte subpopulationsa. Frozen serum/plasma: Hepcidinb, TfRb, adiponectina, resistina, leptin, interleukinsa, phospholipid profilesa, lipid peroxidationa, tocopherola, retinola.	24 wk, 34 wk, delivery (mother and cord blood)
Perinatal records	Gender, gestational age, Apgar, delivery mode, neonatal diagnoses/admission	–
Infant anthropometrics	Birth weight, length, circumference of head, upper-arm, chest and waist, BMI, waist/height index, heel-knee, hip-knee	Birth
Meconium	Microbiotaa	Birth
Breast milk	Fatty acidsa, vitaminsa	day 1, day 7, day 30

^aUnpublished data

^bPublished elsewhere