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. 2016 Mar 1;16:25. doi: 10.1186/s12896-016-0255-z

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Detection of rpIGF-I₃ by SDS–PAGE (a) and Western blot (b) analysis of cell lysates of L. lactis NZ9000 (pNZ8148-pIGF-I₃) induced with nisin. a Lanes 1 and 2, cell lysates of L. lactis NZ9000 (pNZ8148-pIGF-I₃); lanes 3 and 4, cell lysates of L. lactis NZ9000 (pNZ8148); lane 5, molecular weight marker. b Lanes 1 and 3, cell lysates of L. lactis NZ9000 (pNZ8148); lanes 2 and 4, cell lysates of L. lactis NZ9000 (pNZ8148-pIGF-I₃)