Fig. 7.

novel miRNAs in rainbow trout: prediction and tissue distribution. a The illustration shows sequence and structure statistics of one predicted miRNA precursor as an example. b The graph shows, as an example, the 54 nt sequence (putative miRNA precursor) along with reads that aligned to this sequence. The secondary structure of the precursor is shown with the orange parenthesis. c Unsupervised average linkage clustering analysis of new miRNA in rainbow trout tissue. Each row represents a new miRNA locus (total of 94 rows) and each column a tissue RNA sample. Data were median-centered prior to the clustering analysis. For each miRNA, the expression level within sample set is indicated using a color density scale