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. 2016 Mar 1;2:4. doi: 10.1186/s40851-016-0040-9

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© Dhungel et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Detection of Dll-gfp mRNA from wings by RT-PCR. a The Dll-gfp cDNA amplified from wings of infected pupae (a boxed band), which corresponds to the predicted cDNA size, 1708 bp. M: λHindIII marker. These two lanes were run together in a single gel. b Dll cDNA amplified from wings of infected and non-infected pupae (boxed bands) corresponding to the predicted cDNA size, 855 bp. Pupal wings were isolated at the post-infection day indicated. Endogenous Dll is amplified in addition to exogenous Dll-gfp. M: λHindIII marker. These four lanes were run together in a single gel. c Semi-quantification of Dll mRNA transcripts (including exogenous and endogenous ones) (n = 3 for each category) from 4-day-old pupal wings. The data shown are mean ± SD