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. 2016 Mar 1;35:39. doi: 10.1186/s13046-016-0314-2

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© Zervos et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Control vector (pcDNA plasmid and retroviral vector [RV]) and mesothelin overexpressing tumors harvested from mice (Fig. 6) were frozen, fixed, and stained with biotin conjugated rat anti-mouse CD8, Vectastain Elite ABC and developed with Vector DAB Peroxidase substrate. Spleen cells (positive control) show strong staining of CD8+ cells compared to negative control (no primary antibody) or tumor tissue sections