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. 2016 Mar 1;35:39. doi: 10.1186/s13046-016-0314-2

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© Zervos et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Mesothelin expression on the cell surface. Panc02 wild type (wt), retrovirus (RV), clones 2, 4, 10; or pcDNA clones 2, 14, and 17, or control null vector cells were incubated with monoclonal antibody B35 (black line) or normal rat IgG (gray line) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Mean fluorescence intensities (MFI) show increased mesothelin in overexpressing clones. RV clones expressed significantly more mesothelin than vector and wild type p < 0.001. pcDNA clones 2 and 14 expressed significantly more mesothelin than vector and wild type p < 0.05