Fig. 2.

Absolute cell counts and cell count ratios in primary versus recurrent GBM. Primary and recurrent GBM pathology specimens were stained for CD3, CD4, CD8 and FoxP3+ markers and quantified using Aperio software. Absolute counts were divided by surface area of each specimen to standardize measurements, and the ratio of CD4, CD8 and FoxP3+ cells was measured over CD3+ cells. Absolute counts alone were not different between primary and recurrent GBM (a, b, c), but when expressed as percentage of T cell subsets over total CD3+ counts, recurrent GBMs had decreased proportions of CD4+/CD3+ cells (d); however, CD8+ and FoxP3+ cells remained unchanged when expressed as a percentage over total CD3+ cells (e, f)