Figure 4.

Effect of miR-202 expression on the invasive ability of esophageal squamous cell carcinoma EC9706 cell lines was investigated using a Transwell® chamber assay. Cells invading through the Matrigel were stained with Giemsa. Random fields of (A) negative control, (B) normal EC9706 cells and (C) EC9706 cells transfected with miR-202 mimics were visualized using an inverted microscope (magnification, ×10). (D) Histograms demonstrated the mean normalized number of cells that successfully invaded the Matrigel layer. *P<0.05 vs. the negative control.