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. 2016 Jan 25;11(3):709–716. doi: 10.3892/etm.2016.3022

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Streptavidin-biotin complex immunohistochemical analysis of AQP4 protein expression levels in the (A) brain cortex, (B) cerebellum and (C) brainstem of the various groups (3,3′-diaminobenzidine; magnification, ×100; 14 days). At least five fields with positive results were identified in each case. Arrows indicate AQP4-positive cells and dark brown indicates strongly positive AQP4 staining. AQP4, aquaporin-4; CK, control group; HH, hypoxia-hypercapnia group; CU, curcumin group; DMSO, dimethyl sulfoxide solvent group; GM1, monosialoganglioside group.