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PAB did not kill all tumor cells after 3 days. MCF-7 cells were treated with 4 µM PAB for 3 days and subsequently stained with SA-β-galactosidase, a marker of senescence. PAB induced partial cell senescence and partially affected cell growth compared with the Con group. The experiments were performed in triplicate. Black arrow indicates senescent cells; white arrow indicates cells with normal morphology. Scale bar, 45 µm. PAB, pseudolaric acid B; Con, control.
