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. 2016 Mar 2;7:227. doi: 10.3389/fpls.2016.00227

FIGURE 2.

FIGURE 2

The resistance in cyp83a1-3 is SA-independent. (A) Four-weeks-old Arabidopsis wild-type, cyp83a1-3 mutant, and double mutant plants were infected with G. cichoracearum, and representative leaves were removed and stained with trypan blue at 8 dpi, bar = 200 μm. (B) Quantification of fungal growth of the plants in (a) at 5 dpi by counting the number of conidiophores per colony. Results represent the mean and standard deviation in three independent experiments (n = 30). Different letters represent statistically significant differences (P < 0.01, nested ANOVA). (C) Free SA levels were measured in the uninfected and infected (3 dpi) leaves after inoculation with G. cichoracearum. FW, fresh weight. (D) Accumulation of PR1, PR2, and FRK1 transcripts in 4-weeks-old plants infected by G. cichoracearum examined by quantitative real-time PCR. Results represent the mean and standard deviation in three independent experiments (n = 4).