FIGURE 3.

Transcript accumulation of genes related to glucosinolate or camalexin biosynthesis upon G. cichoracearum infection. (A) Four-weeks-old wild-type plants were infected with G. cichoracearum, and the accumulation of CYP83A1, SUR2, CYP71A13, and PAD3 transcripts was examined by quantitative real-time PCR. Results represent the mean and standard deviation in three independent experiments (n = 4). (B) Four-weeks-old CYP83A1-GFP transgenic plants were infected with G. cichoracearum and immunoblot analysis was performed using an anti-GFP antibody. The large subunit of Rubisco was used as a protein loading control.