Skip to main content
. 2016 Mar 2;7:227. doi: 10.3389/fpls.2016.00227

FIGURE 4.

FIGURE 4

The cyp83a1-3 mutant accumulates high levels of camalexin upon G. cichoracearum infection, which is suppressed by mutation of PAD3. (A) Four-weeks-old wild-type, cyp83a1-3, pad3 mutant, and double-mutant plants were infected with G. cichoracearum. Representative leaves were removed and stained with trypan blue at 8 dpi, bar = 200 μm. (B) Quantification of fungal growth of the plants in (a) at 5 dpi by counting the number of conidiophores per colony. Results represent the mean and standard deviation in three independent experiments (n = 30; P < 0.01, nested ANOVA). (C) Four-weeks-old plants were infected with G. cichoracearum. Camalexin accumulation was determined at 0 and 5 dpi. Results represent the mean and standard deviation in three experiments (n = 3). Asterisk represents statistically significant difference from wild-type (P < 0.01, nested ANOVA). (D) The transcript accumulation of PAD3 was examined by quantitative real-time PCR on samples from 4-weeks-old wild-type, cyp83a1-3 and pad3 mutant plants. (E) The transcript accumulation of CYP71A13 examined by quantitative real-time PCR. Results represent the mean and standard deviation in three independent experiments (n = 4).