Table II.
List of primers used for reverse transcription-quantitative polymerase chain reaction.
| Gene | Primer sequence (5′-3′) |
|---|---|
| TGF-β | F: TTGCTTCAGCTCCACGGAGAA |
| R: ACGTAGTACACGATGGGCAGC | |
| CXCL10 | F: CGCTGTACCTGCATCAGCATTAG |
| R: CTGGATTCAGACATCTCTTCTCACC | |
| PTGS2 (COX2) | F: ACTATGGCTACAAAAGCTGGGAAG |
| R: ATCATCAGGCACAGGAGGAAG | |
| VEGFA | F: CAGGAGTACCCTGATGAGATCG |
| R: TCTGCATGGTGATGTTGGAC | |
| IL-6R | F: CACGACTCTGGAAACTATTCATGC |
| R: AGGACCCCACTCACAAACAAC | |
| CCL2 | F: GTGTCCCAAAGAAGCTGTGATCT |
| R: TGTCCAGGTGGTCCATGGA | |
| OSM | F: GCCCAGGATTTGGAGAGGTCTGG |
| R: GCGATGGTAGCCATGCAGGAACCT | |
| β-actin | F: ATTCCTATGTGGGCGACGAG |
| R: AAGGTGTGGTGCCAGATTTTC |
F, forward; R, reverse; TGF, tumor growth factor; CXCL10, chemokine (C-X-C motif) ligand 10; PTGS, prostaglandin-endoperoxide synthase; COX; cyclooxygenase; VEGF, vascular endothelial growth factor; IL; interleukin; CCL2, chemokine (C-C motif) ligand 2; OSM, oncostatin M.