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Effects of recombinant S100A8/A9 protein on GATA3 and ESR1 gene expression in MCF-7 cells. Cells were treated with 10 ng/ml S100A8/A9 for 24 h, while control cells received no treatment. (A) GATA3 and (B) ESR1 mRNA levels were measured using quantitative polymerase chain reaction. Data are expressed as the mean ± standard error. n=4/group. ***P<0.001 vs. control. GATA3, GATA binding protein 3; ESR1, estrogen receptor 1; mRNA, messenger RNA; NS, not significant.
