Figure 5.

Effects of Lcn-2 overexpression on miR-138-induced cell proliferation. (A) Protein expression levels of Lcn-2 in PC12 cells, as assessed by western blot analysis. PC12 cells were harvested for western blot analysis at 72 h after transfection with miR-138 mimics (100 nM) + pcDNA3.1 (500 nM) or miR-138 mimics (100 nM) + pcDNA3.1-Lcn-2 (500 nM). (B) Cell growth rate was determined by MTT assay, and time-dependent changes are presented as the mean ± standard deviation and analyzed by one-way analysis of variance (*P<0.05 vs. miR-138 + Lcn-2). Lcn-2, lipocalin 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; miR, microRNA; MTT, methyl thiazolyl thiazolium.