Fig. 2.

Aloin increased the ALP activity in MC3T3-E1 cells (A) and human adult adipose derived stem cells (hADSC) (B). Cells treated with aloin were fixed and incubated in ALP substrate buffer for 30 min. Theabsorbance at 405 nm was measured as the p-nitrophenol released in nmol using a microplate reader Valueson y-axis represents the nmol ALP production (nmol/ugm protein). The data represent mean ± SD of threeexperiments. ^*p<0.05 vs control cells. (C) MC3T3-E1 cells treated with 0.05 μM aloin for 10 days were stained for ALP content. ALP in the form of blue droplets were monitored in microscope and photographed using Olympus microscope (Model # 1X73).